Document Type : Research Paper

Authors

• hadis Mirzaei ¹
• ali Aghaei ²
• elham hoveizi ³
• mahmood nazari ⁴

¹ PhD student,, Department of Animal Science, Faculty of Animal Science and Food Technology, Agricultural Science and Natural Resources University of Khuzestan, Mollasani, Iran

² Assistant Professor, Department of Animal Science, Faculty of Animal Science and Food Technology, Agricultural Science and Natural Resources University of Khuzestan, Mollasani, Iran

³ Associate Professor, Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz.iran

⁴ Associate Professor, Department of Animal Science, Faculty of Animal science and Food Technology, Agricultural Science and Natural Resources University of Khuzestan, Mollasani, Ahvaz, Iran

Abstract

Background and Objective: Heat stress can lead to oxidative stress, as a result, it affects the immune status, health, and function of living cells. On the other hand, the antioxidant properties of alpha-lipoic acid and the role of chromium in glucose metabolism are associated with reducing the effects of oxidative stress. Tantioxidant status and the immune response of cultured cells under heat stress.

Materials and Methods: In the present study, BMSCs were isolated from day-old chicks of Ras 308 breed. To differentiate BMSCs into fat and bone cells, they were treated with adipogenic and osteogenic medium, respectively. Experimental groups include cells without any treatment, cells that suffered heat shock, and heat-shocked cells treated with 100 μM chromium propionate and 100 μM alpha-lipoic acid separately and simultaneously. Then, BMSCs were compared in terms of morphology, survival, measurement of antioxidant factors, and the expression level of genes related to immunity. Data were analyzed through SAS software.

Results: The isolated cells were morphologically fibroblastic and identified using flow cytometry and differentiation potential. The highest level of activity of SOD, CAT, GPX, and TAC enzymes was observed in the alpha-lipoic acid group under heat shock. The lowest levels of IL-2, IL-6, and INF-γ gene expression were observed in the group of cells treated simultaneously with chromium propionate and alpha-lipoic acid and in the group of cells treated with alpha-lipoic acid, respectively.

Conclusion: The results showed that the use of antioxidant supplements in heat shock conditions improves antioxidant and immune responses.

Keywords

• Mesenchymal Stem Cells
• alpha-lipoic acid
• chromium
• immune response
• antioxidant status

Main Subjects

• Developmental