Document Type : Research Paper

Authors

• Salimeh Asadi ¹
• Hamidreza Ahmadniaye motlagh ²
• Omid Safari ³
• hamed vardast zadeh ⁴
• Yasman Zeraatpisheh ¹
• Ali Javadmanesh ⁵

¹ Department of Animal science, Faculty of Agriculture, Ferdowsi University of Mashhad, Razavi Khorasan, Iran

² Assistant Professor Faculty of Natural Resources and Environment Ferdowsi University Of Mashhad

³ Department of fisheries, Faculty of Natural Resources and Environment, Ferdowsi University of Mashhad, Razavi Khorasan, Iran.

⁴ Department of Fisheries, Faculty of Natural Resources and Environment, Ferdowsi University of Mashhad, Razavi Khorasan, Iran

⁵ Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad

Abstract

This study was designed for the first time to compare the effects of different levels of apple cider vinegar on the growth performance and expression of antioxidant genes (catalase and glutathione peroxidase) in rainbow trout (Oncorhynchus mykiss). For this purpose, a herd of 300 animals (73.35 ± 14.24 g) was fed with a special ration for inducing fatty liver, which was adjusted for the first time, for one month; Then, 120 infected fish (74.20 ± 5.40) were selected from the intended flock and transferred to the respective cages. This experiment was conducted as a completely randomized design with six treatments and three replications. Experimental treatments include: treatment 1: healthy fish without apple cider vinegar, treatment 2: healthy fish with four percent apple vinegar, treatment 3: infected fish without apple vinegar, treatment 4: infected fish with one percent apple vinegar, treatment 5: infected fish with Two percent apple vinegar and treatment 6: infected fish with four percent apple vinegar. Feeding was done daily at the rate of two percent of body weight for 60 days. Based on the results, no difference was observed between different treatments in terms of final weight. Also, the results of gene expression showed that apple cider vinegar had no significant effect on catalase and glutathione peroxidase genes. In conclusion, ACV administration showed no significant effect on the antioxidant activity of rainbowtrout with non-alcoholic fatty liver disease, but improved growth function of treated fish.

Keywords

• Acetic acid
• Catalase
• Fatty liver
• Glutathione peroxidase