Document Type : Research Paper

Authors

• sevda zarei ¹
• Hossein Ghafouri ¹
• leila vahdatiraad ¹
• tooraj sohrabi ²
• behrooz heidari ¹

¹ Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran

² International Caspian Sturgeon Research Institute, Agricultural Research; Education and Extension Organization (AREEO), Rasht, Iran

Abstract

Introduction: Cortisol is one of the most significant factors contributing to controlling stress. Additionally, another well-known mechanism for protecting cells against various stresses and linked to cortisol is the reaction to heat shock, which triggers the production of heat shock proteins (HSP). By strengthening sturgeon fish's defense system against environmental stress, such as temperature stress, their stocks can be preserved.

Materials and methods: In this study, sterlet sturgeon (Acipenser ruthenus) liver tissue cells were extracted and cultured in flasks. Nopal Endurance (Pro-Tex®), amygdalin, and pyrazole derivatives (SZ) were used as HSP inducers (HSPi). In order to determine the viability and optimal dose, MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-2H-tetrazolium bromide) assay was performed. The activity of cortisol and biochemical enzymes was analyzed in liver cells under temperature stress (18, 22, and 26 °C) in the presence and absence of HSPi compounds.

Results: MTT assay showed that cells receiving HSPi compounds increased survival. They could increase cortisol levels alone or in combination with stress. Amygdalin reduced biochemical enzyme activity.

Discussion and conclusion: Escape from apoptosis under stress conditions is a basic strategy for cell survival. Considering the role of cortisol and its relationship with HSP in environmental stress conditions, it can be concluded that treating cells with HSPi compounds can make cells resistant to stress and the harmful effects caused by stress reverse and ultimately increase survival.

Keywords

• Apoptosis
• Amygdalin
• Cell Stress
• Heat Shock Protein
• Sturgeon

Main Subjects

• Biochemistry