Document Type : Research Paper

Author

• ghodrat ebadimanas

Department of Biology, Faculty of Basic Sciences, Farhangian University, Tehran, Iran

Abstract

Today one of the most important challenges for humans is environmental pollution through agricultural toxins, especially insecticides. Spirotetramat is a new insecticide that is widely used by farmers, and its side effects on the reproductive system have not been well studied. The aim of this study was to investigate the effect of spirotramate on oxidative stress and histochemical changes of testicular tissue in mice. In this study, 24 adult male mice, with a body weight range of 24-32 grams, were obtained from Urmia University Veterinary Animal House and studied. The animals were randomly divided into three group: controls, test group 1 and test group 2. Each group included 8 mice. Test group 1 and 2 received the spirotetramat at rats of 2.5 and 10 mg/kg body weight, respectively, and the control group received the same amount of distilled water for 45 days orally by gavage. After this period, the mice were anesthetized and microscopic sections were prepared. The level of oxidative stress, carbohydrate reserves, cytoplasmic fat reserves and the level of inflammation in testicular tissue were investigated. Qualitative histochemistry findings were converted into quantitative data using Imag-J software. The results showed that spirotetramat decreased carbohydrate reserves, TAC, TTM and increased fat reserves, alkaline phosphatase enzyme, NO and MDA in the experimental groups compared to the control group. Therefore spirotetramat has increased the oxidative stress and histochemical changes in the testicular tissue by damaging the cells of the spermatogenic tube, as well as by inhibiting the activity of acetyl coenzyme-A.

Keywords

• Histochemical
• oxidative stress
• Spirotetramat
• Testis
• Balb/c Mice

Main Subjects

• Histology