Document Type : Research Paper

Authors

• Amir Emami ¹
• Ahmad Ali Mohammadpour ²

¹ Department of Basic Sciences, Faculty of Veterinary medicine, Ferdowsi University of Mashhad

² Department of Basic Sciences, Faculty of Veterinary medicine, Ferdowsi University of Mashhad, Mashhad, Iran

Abstract

The anatomical structure of the eye, the type of lacrimal glands and its secretions can be important in the absence of some damage to the eye. Five healthy male cockatiels were used in this research.

After dissecting the specimens and detrmining the location of the gland,tissue sections were prepared using usual tissue preparation methods and stained with hematoxylin-eosin, PAS, Alcian blue and toluidine blue. The results showed that Harderin's gland in the cockatiel is triangular and located in the inner ventral surface of the eyeball. Its average length was 4.5 mm and its average width was 3.14 mm. In tissue sections, it was observed that this gland consists of several lobes that are surrounded by a thin capsule with fine threads called septa extend into the gland and divide it into lobules. The shape of the secretory units of Harderin gland was compound tubular alveolar type and the covering tissue of its wall was simple columnar type. The secretions of Harderin gland were stained with PAS and Alcian blue stains, which showed a significant response to Alciain blue staining in the results of secretory cells of the gland and due to the presence of acidic mucins in the cells of the secretory unit and ducts of these structures. They showed a positive reaction to this stain, but due to the small amount of neutral mucin compounds, the secretory cells of this gland showed a weak reaction to PAS staining and the secretions were observed as pale purple.

Keywords

• Histomorphology
• Harderian gland
• Cockatiel

Main Subjects

• Histology