Document Type : Research Paper

Authors

• Behnam Poureslamfar ¹
• Behzad Fathinia ¹
• Arya Shafaeipour ²
• Hossein Farasat ³

¹ Department of Biology, Faculty of Science, Yasouj University, Yasouj, Iran

² Yasouj University

³ Department of Science, Farhangian University, Tehran, Iran.

Abstract

In this study, 67 adult tree frogs, Hyla savignyii, were taken from Dasht-e-Poune and Paddy fields in Lodab region from May 2021 to November 2022, to investigate the hematology of the tree frogs. 11 metric characters were measured using a digital caliper (0.01 mm accuracy) to examin the morphology of male and female frogs. Blood samples were taken from the abdominal vein, smears were prepared, dried at ambient temperature, fixed with methanol, and stained by Giemsa. The blood cells (erythrocytes, leukocytes, and thrombocytes) were investigated under 40x and 100x magnification using an optical microscope. The male and female groups separate from each other in five out of 11 metric characters (p≤0.05). All the six metric characteristics of red blood cells in female frogs are greater than male frogs. Leuckocyte characters are significantly different between sexes with greater values for female group (p≤0.05). Different environmental and gender factors may responsible for cell metric differences between sexes.

Keywords

• red blood cells
• white blood cells
• paddy fields
• dasht-e-poune
• hematology

Main Subjects

• Biosystematic